Medi PT reagent kit for prothrombin time determination

Medi PT reagent kit for prothrombin time determination
Stock: In stock
Price: On request

Medi PT reagent / Меди ПТ

Medi PT reagent reagent kit for prothrombin time determination

Medi PT reagent reagent kit / Меди ПТ

Highly sensitive stable lyophilized thromboplastin with calcium for determination of prothrombin time (BioMedica Diagnostics Inc., Canada)

Principle of the method

consists of determining the clotting time of citrated plasma after adding a thromboplastin-calcium mixture to it. The method characterizes the activity of factors of the “extrinsic” coagulation pathway (II, V, VII, X and fibrinogen)

Kit composition: Can be formed into kits of any size

Medi PT release forms:

  • 1 vial x 4 ml (20/40 tests)
  • 1 vial x 10 ml (50/100 tests)
  • Set of 10 vials x 4 ml (200/400 tests)
  • Set of 10 vials x 10 ml (500/1000 tests)

DO NOT FREEZE AFTER DILUTION!

Forms of presentation of results: prothrombin time (sec), prothrombin index (%), prothrombin ratio, Quick percentage (%), INR (international normalized ratio)

Prothrombin time determination method:

  1. Add 50/100 μl of the test plasma to the reaction cuvette, keep at +37℃ for 100 seconds
  2. Add 100/200 μl of MediPT reagent preheated to +37°C and determine the clotting time

Indications for use:

  • screening of the hemostasis system
  • monitoring of oral anticoagulant therapy
  • vitamin K deficiency
  • diagnosis of liver function

Sensitivity:

In order to increase the accuracy of determination INR for the MediPT reagent is supplied with MIC (0.98 -1.29) for different types of coagulometers, for example: optical method – MIC 1.09, average prothrombin time – 12.8 sec; Mechanical method – MIC 1.17, average prothrombin time – 12.1 sec.

Storage stability:

8 days (2÷8 ºС) change in sensitivity up to 1.5%
32 hours (37 ºС) change in sensitivity up to 1.0%
48 hours (24 ºС) change in sensitivity up to 1.8%
Shelf life
of undiluted reagent (2÷8 ºС)
3 years

Reproducibility of studies:

In the study CV = 0.27%
«vial-to-vial» CV = 0.51%
«day-to-day» CV = 2.50%
«series-to-series» CV = 3.43%

Adaptation of a reagent for determining prothrombin time (download)